Effect of berberine, liensinine and neferine on HERG channel expression.
- Author:
Ting WEI
1
;
Zhe LIANG
;
Yan JIN
;
Li ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Anti-Arrhythmia Agents; analysis; pharmacology; Arrhythmias, Cardiac; drug therapy; Benzylisoquinolines; analysis; pharmacology; Berberine; analysis; pharmacology; Blotting, Western; Dose-Response Relationship, Drug; ERG1 Potassium Channel; Ether-A-Go-Go Potassium Channels; drug effects; metabolism; Fluorescent Antibody Technique; Gene Expression Regulation; drug effects; HEK293 Cells; Humans; Isoquinolines; analysis; pharmacology; Male; Phenols; analysis; pharmacology; Rats
- From: China Journal of Chinese Materia Medica 2013;38(2):239-244
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEImmunofluorescence and Western blot methods were adopted for qualitative and quantitative detections of the effect of different concentrations of berberine, liensinine and neferine on the expression of stable transfection in HERG potassium channel in HEK-293 cells, as well as the effect of different concentrations of berberine on protein expression of Ikr channel in cardiac muscular tissues, in order to investigate the anti-arrhythmic mechanism of berberine, liensinine and neferine.
METHODWestern blot method was used to detect protein expression of HERG channel in HERG-HEK cells. Immunofluorescence method as well as confocal laser microscope were used to detect the effect of different concentrations of berberine, liensinine and neferine on protein expression of HERG channel. Western blot method was used to detect the effect of different concentrations of berberine on protein expression of Ikr channel in cardiac muscular tissues as well as the effect of berberine, liensinine and neferine on protein expression of stable transfection in HERG potassium channel in HEK-293 cells.
RESULTWestern blot experiment manifested that stable transfection of HEK293 cells containing HERG genes could increase protein expression of HERG channel. Berberine (10, 30 micromol x L(-1)) remarkably inhibited protein expression of HERG channel in HERG-HEK cells (P < 0.01). Berberine (10, 20 mg x kg(-1)) also inhibited protein expression of Ikr channel in rat ventricular tissues (P < 0.05). Liensinine (3, 10, 30 micromol x L(-1)) increased protein expression of HERG channel in HERG-HEK cells (P < 0.05). Neferine showed no effect on protein expression of HERG channel in HERG-HEK cells.
CONCLUSIONThe stably transfection of HERG-HEK cells can increase protein expression of HERG channel. Berberine shows inhibitory effect on protein expressions of in vitro HERG-HEK cells and Ikr channel in rat ventricular tissues. Liensinine improves protein expression of HERG channe in HERG-HEK cells. Neferine shows no effect on protein expression of HERG channel.
