Determination of naringenin in Dendrobium officinale by HPLC.
- Author:
Gui-Fen ZHOU
1
;
Su-Hong CHEN
;
Gui-Yuan LV
;
Mei-Qiu YAN
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; Dendrobium; chemistry; growth & development; Flavanones; analysis; Medicine, Chinese Traditional; Methanol; chemistry; Reproducibility of Results; Water; chemistry
- From: China Journal of Chinese Materia Medica 2013;38(4):520-523
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore a characteristic chemical marker of Dendrobium officinale, establish determination method of its content and determine the naringenin content in D. officinale from different sources and growth years.
METHODThe content of naringenin was determined by HPLC. HPLC analysis was made on a XB -C18 (4.6 mm x 250 mm, 5 microm) with methanol and water containing 0.2% phosphoric acid as mobile phase. The detection wavelength was 290 nm.
RESULTThe HPLC method showed good linearity within the range of 0.026-0.208 microg (r = 1). The average recovery of naringenin was 96.3% (RSD 1.8%). The naringenin content was the highest in 3 years D. officinale and had some differences from different sources.
CONCLUSIONThe method is accurate and reliable. It is appropriate for the quantitative determination of naringenin in D. officinale and it's production.
