Protective effects of polysaccharides from Dendrobium huoshanense on CCl4-induced acute liver injury in mice.
- Author:
Jing HUANG
1
;
Sheng-Li LI
;
Hong-Wei ZHAO
;
Li-Hua PAN
;
Hao-Qiao SUN
;
Jian-Ping LUO
Author Information
- Publication Type:Journal Article
- MeSH: Alanine Transaminase; blood; Animals; Aspartate Aminotransferases; blood; Carbon Tetrachloride; toxicity; Chemical and Drug Induced Liver Injury; blood; enzymology; pathology; prevention & control; Dendrobium; chemistry; Gene Expression Regulation; drug effects; Liver; drug effects; metabolism; pathology; Male; Malondialdehyde; metabolism; Mice; Polysaccharides; chemistry; pharmacology; Superoxide Dismutase; metabolism; Tumor Necrosis Factor-alpha; metabolism
- From: China Journal of Chinese Materia Medica 2013;38(4):528-532
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the protective effects of polysaccharides from Dendrobium huoshanense (DHP) against CCl4-induced liver injury in mice.
METHODEighty male Kunming mice were randomly divided into normal control group, model control group, dextran control group, starch control group, hydrolyzate control group, three different dose of DPH groups consisting of high-dosage group, middle-dosage group and low-dosage group (200, 100, 50 mg x kg(-1)). Each group contained ten mice. The mice were treated with DHP via intragastric administration for 15 days before treatment of 50% CCl4 in olive oil for consecutive two days. Both alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities in serum and superoxide dismutase (SOD) activities and malondialdehyde (MDA) contents in liver tissues were determined in all groups. Immunohistochemistry was used to detect the expression of TNF-alpha in hepatic tissue. Hepatic histopathological examination was observed.
RESULTDHP effectively decreased the activities of ALT and AST in serum and the contents of hepatic MDA, and restored hepatic SOD activities in acute liver injury mice. Liver tissue damage induced by CCl4 was ameliorated in mice with DHP administration through histopathology examination. Furthermore, the expression of TNF-alpha was greatly decreased in groups treated with polysaccharides.
CONCLUSIONDHP has a significantly hepatoprotective effect on CCl4-induced acute liver injury in mice. Protective effect of DHP on the liver may be related to its function of scavenging free radicals and inhibiting lipid peroxidation and TNF-alpha expression.
