Optimization of extraction process of Chrysanthemi Flos by multi-index orthogonal experiment.
- Author:
Xing-Hai ZHANG
1
;
Huan LI
;
Kun-Ming QIN
;
Xiao LIU
;
Bin WANG
;
Bao-Chang CAI
Author Information
- Publication Type:Journal Article
- MeSH: Chemical Fractionation; methods; Chrysanthemum; chemistry; Drugs, Chinese Herbal; chemistry; isolation & purification; Ethanol; chemistry; Hot Temperature; Quality Control; Reproducibility of Results; Solubility; Time Factors
- From: China Journal of Chinese Materia Medica 2013;38(6):821-824
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore quantitative assessment indicators of Chrysanthemi Flos, and optimize the extraction process of Chrysanthemi Flos through orthogonal experimental design.
METHODThe concentration of ethanol, amount of ethanol, extraction time and extraction frequency were selected as factors in the L9 (3(4)) orthogonal experiment. A comprehensive assessment was conducted with the peak area of the eight major common peaks in the fingerprint of Chrysanthemi Flos as the indicators.
RESULTThe optimum extraction process was selected as follows: using ultrasonic extraction method, adding 30-fold ethanol with 80% concentration, extracting for 2 times for extraction, 40 min for each time.
CONCLUSIONThe optimized extraction process is reliable, with controllable assessment indicators, which is significant to the standardization of the extraction process and quality control of Chrysanthemi Flos preparations.
