Determination of triterpenoic acids in fruits of Ziziphus jujuba using HPLC-MS with polymeric ODS column.
- Author:
Yong ZHANG
1
;
An ZHOU
;
Xiao-Mei XIE
Author Information
1. School of Pharmacy, Anhui College of Traditional Chinese Medicine, Hefei 230031, China. yongzh@163.com
- Publication Type:Journal Article
- MeSH:
Chromatography, High Pressure Liquid;
methods;
Fruit;
chemistry;
Mass Spectrometry;
methods;
Polymers;
chemistry;
Triterpenes;
analysis;
isolation & purification;
Ziziphus;
chemistry
- From:
China Journal of Chinese Materia Medica
2013;38(6):848-851
- CountryChina
- Language:Chinese
-
Abstract:
A simple and sensitive method has been developed to simultaneously determine betunilic acid, oleanolic acid and ursolic acid in the fruits of Ziziphus jujuba from different regions by HPLC-MS. This HPLC assay was performed on PAH polymeric C18 bonded stationary phase column with mobile phase contained acetonitrile-water (90: 10) and with negative ESI detection mode. The developed approach was characterized by short time consumption for chromatographic separation, high sensitivity and good reliability so as to meet the requirements for rapid analysis of large-batch fruits of Z. jujuba from different habitats.
