HPLC characteristic chromatographic profile of Sarcandra glabra.
- Author:
Pei-Ling LIN
1
;
Jian-Wei ZENG
;
Chun-Hua DING
;
Yi-Chi LIANG
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; Cluster Analysis; Drugs, Chinese Herbal; chemistry; standards; Ferns; chemistry; Organic Chemicals; analysis; chemistry; isolation & purification; Quality Control
- From: China Journal of Chinese Materia Medica 2013;38(6):856-860
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop the characteristic chromatographic profile of Sarcandra glabra by HPLC for its quality control.
METHODThe HPLC analysis was performed on an Agilent Zorbax Eclipse XDB-C18 column (4. 6 mm x 250 mm, 5 microm) with column temperature at 40 degree C. The mobile phase was consisted of water containing 0. 5% formic acid and acetonitrile to methanol (1:9) in gradient mode, and the detection wavelength was set at 344 nm.
RESULTA common mode of the HPLC characteristic chromatographic profile has been establised. There were 20 common peaks , seven of which were identified, and 46 samples from different habitats were classified into five groups based on principal component cluster analysis.
CONCLUSIONThe method was time-saving and can represent the chemical information and provide a scientific basis for quality control of S. glabra.
