Experimental study on polysaccharide of Cipangopaludina chinensis against HBV in vitro.
- Author:
Xiao-Yan LIU
1
;
Chao-Pin LI
;
Ke-Xia WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antiviral Agents; adverse effects; pharmacology; DNA, Viral; metabolism; Hep G2 Cells; Hepatitis B Surface Antigens; metabolism; Hepatitis B e Antigens; metabolism; Hepatitis B virus; drug effects; metabolism; Humans; Polysaccharides; adverse effects; pharmacology; Snails; chemistry
- From: China Journal of Chinese Materia Medica 2013;38(6):879-883
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the biological activity of polysaccharide of Cipangopaludina chinensis (PCC) against HBV in vitro.
METHODHepG2 2. 2. 15 cells were taken as the in vitro experimental model. The cell toxicity was observed by MTT. PCC of different safe concentrations and positive control medicine 3TC were added into the cells. Cell control without medicine was set at the same time. Cultural supernatants were collected at 9 d. HBsAg and HBeAg in cultural supernatants were tested by ELISA. The content of HBV-DNA was detected by TaqMan probe fluorescence quantitative PCR.
RESULTTC0 and TC50 of PCC in HepG2 2. 2. 15 cell culture were 1 g . L-1 and >10 g . L-1, respectively, suggesting low toxicity in cells. IC50 of PCC in HepG2 2. 2. 15 cells HBsAg and HBeAg were 0. 501, 0. 401 g. L-1, with SI being >19.96 and >24. 94, respectively. PCC could effectively inhibit the secretion of HBsAg and HBeAg, and have a better effect on HBeAg than on HBsAg. PCC had a significant inhibitory effect on HBV-DNA in HepG2 2. 2. 15 cells at concentrations of 0. 1, 1 g . L-1 P <0.05).
CONCLUSIONPCC has the effect against HBV activity in vitro to some extent, with low toxicity, thereby having a good prospect for application.
