Pulsed-field gel electrophoresis typing on non-O157 Shiga toxin-producing Escherichia coli isolates.
- Author:
Dong JIN
1
;
Ai-lan ZHAO
;
Xiang-ning BAI
;
Qiong MENG
;
Bo YU
;
Xue-jiao YUAN
;
Yan-wen XIONG
;
Xue-xin HOU
;
Zhen-jun LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; China; epidemiology; DNA, Bacterial; Electrophoresis, Gel, Pulsed-Field; Escherichia coli Infections; epidemiology; microbiology; Escherichia coli O157; genetics; isolation & purification; Feces; microbiology; Genotype; Humans; Shiga-Toxigenic Escherichia coli
- From:Chinese Journal of Epidemiology 2013;34(5):503-506
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a database and to understand the molecular epidemiological features of non-O157 Shiga toxin-producing Escherichia coli (STEC) isolates from different animal reservoirs and patients.
METHODSPulsed-field gel electrophoresis (PFGE) was performed according to the PulseNet protocol with minor modifications. A dendrogram was constructed using the BioNumerics.
RESULTSUnder the PulseNet protocol, 62 PFGE patterns were obtained from 76 non-O157 STEC isolates and then divided into A to M groups. Isolates from different sources were widely distributed in different groups, but were predominant seen in certain groups.
CONCLUSIONThe non-O157 STEC isolates in China were highly polymorphic. PulseNet protocol seemed to be suitable for the typing of Chinese non-O157 STEC isolates.
