Effects of allergen and intranasal glucocorticoid on Th17 and RORγt in peripheral blood in patients with allergic rhinitis
10.3760/cma.j.issn.1673-0860.2009.12.008
- VernacularTitle:变应原和糖皮质激素对变应性鼻炎患者外周血Th17细胞及其转录因子RORγt的作用
- Author:
Shen-Hong QU
1
;
Min LI
;
Yong-Jian HUANG
;
Zhi-Ying OU
;
Zhi-Bin LIN
;
Jian-Ping LIANG
;
Wen-Sheng LU
Author Information
1. 广西壮族自治区人民医院
- Keywords:
Rhinitis;
allergic perennial;
Allergens;
Immunotherapy;
Glucocorticoids;
Receptors;
retinoic acid;
Receptors;
thyroid hormone;
T-lymphocytes;
helper-inducer
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2009;44(12):996-1000
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of specific immunotherapy and intranasal glucocorticoid on T help 17(Th17)cells and RORγt in peripheral blood in patients with allergic rhinitis(AR).Methods Forty patients with allergic rhinitis(group A)were divided randomly into two subgroups(group A1 and A2),and each subgroup had 20 patients.The patients in group A1 were treated with intranasal glucocorticoid(INGS)for one-year.The patients in group A2 were treated with special immunotherapy (SIT)for 4 weeks.Blood samples were respectively taken from 10 healthy individuals(group B),20 AR patients(group A1)before and after SIT with specific standardized allergen and 20 AR patients(group A2)before and after INGS.The ratio of Th17 cells in peripheral blood monouclear cells(PBMC)were analysed by flow cytometry.The expression of RORγt mRNA were detected by real-time polymerase chain reaction and the interleukin-23(IL-23),IL-17,IL-6 were detected by enzyme-linked immunosorbent assay.Results The ratio of Th17 cells in PBMC and the expression of RORγt mRNA in group A[(18.97 ± 1.05)% and (0.604 ± 0.027)]were respectively higher than those in group B[(15.12 ± 1.09)% and(0.447 ±0.024)]and the difference reached statistical significance(t were respectively-10.056 and-17.986,each P＜0.01).The level of IL-6,IL-17 and IL-23 in group A were respectively higher than those in group B and the difference reached statistical significance(t were respectively-41.149,-17.618 and -26.824,all P＜0.01).The ratio of Th17 cells in PBMC,the expression of RORγt mRNA,the level of IL-6,IL-17 and IL-23 before INGS did not show significant difference from those of after INGS in group A1 (t were respectively 0.298,0.240,-1.136,0.283 and-1.670,all P＞0.05).The ratio of Th 17 cells in PBMC and the expression of RORγt mRNA were respectively(18.99 ± 1.14)% and(0.603 ± 0.027)before SIT and were respectively(16.30 ± 1.63)% and(0.429 ± 0.023)after SIT in group A2,and the difference reached statistical significance(t were respectively 6.035 and 22.015,all P＜0.01).The level of IL-6,IL-17 and IL-23 before SIT were lower respectively than those of after SIT in group A2 and the difference reached statistical significance(t were respectively 9.235,11.289,7.267,all P＜0.01).Conclusions The ratio of Th17 cells in PBMC,the expression of ROR-yt mRNA,the level of IL-6,IL-17 and IL-23 were up-regulated in patients with AR.The treatment of SIT could get the 5 items down and the treatment of INGS couldn't.
