Effect of early allergen immunization on the development of allergic rhinitis in mouse
10.3760/cma.j.issn.1673-0860.2010.01.013
- VernacularTitle:早期变应原免疫对小鼠后期变应性鼻炎影响的研究
- Author:
Yue ZHOU
1
;
Jian-Jun CHEN
;
Zhong-Yuan LU
;
Wei-Jia KONG
Author Information
1. 华中科技大学同济医学院附属协和医院
- Keywords:
Allergens;
mice;
Rhinitis,allergic,perennial
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2010;45(1):52-55
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of early allergen exposure on later development of allergic rhinitis in mouse. Methods Twenty-four BALB/c neonates were randomly divided into 4 groups (low-dose group, high-dose group, negative control group and positive control group), each group had 6 mice. The mice were administered ovalbumin (OVA) by subcutaneous injection on day 1,5, 12 after birth (10 μg OVA in 0.05 ml saline for low-dose group, 1000 μg OVA in 0.05 ml saline for high-dose group,only saline for negative and positive control group).Then the mice were sensitized and intranasally challenged with OVA(saline without OVA was used in negative control group) after 6 weeks. Symptoms,histopathological changes of nasal mucosa were observed, OVA-IgE in serum was examined, cytokines IL-4,IL-5 and IFN-γ were detected in the supernatant of cultured splenic mononuclear cells. Results Compared to the positive control group, symptoms and nasal mucosa histological changes of high-dose group was indistinctive. The level of OVA-IgE and cytokines IL-4, IL-5 ((x)±s) in high-dose group [(265.11±26.29), (446.39±72.83) and (171.24±15.66) pg/ml, respectively] were significantly lower than those in positive control group [(665.85±43.15), (1113.45±30.47), (255.36±30.96) pg/ml,respectively, t value were 0.000, 0.000 and 0.009, respectively, all P<0.05]. The level of IFN-γ in high-dose group [(319.74±56.30) pg/ml] was significantly higher than those in positive control group [(170.02±14.50) pg/ml, t=0.000, P<0.05]. There was no significant difference of the results between the low-dose group and positive control group. Conclusions Neonatal immunization with high-dose OVA inhibited the future allergic rhinitis symptoms, nasal histological changes, serum OVA-IgE levels and Th1/Th2 cytokine imbalance, resulting in the protective effect.
