Effects of double-stranded RNA poly(I:C)on matrix metalloproteinase mRNA expression in human nasal polyp epithelial cells
10.3760/cma.j.issn.1673-0860.2010.03.013
- VernacularTitle:双链RNA对人鼻息肉上皮细胞中基质金属蛋白酶mRNA表达的影响
- Author:
Ji-Yun WANG
1
;
So WATANABE
;
Satoshi MATSUKURA
;
Harumi SUZAKI
Author Information
1. 浙江省宁波市第二医院
- Keywords:
Sinusitis;
Nasal polyps;
Epithelial;
Birnaviridae infections;
Matrix metalloproteinases;
Reverse tranacriptase polymerase chain reaction
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2010;45(3):229-232
- CountryChina
- Language:Chinese
-
Abstract:
Objective Chronic rhinosinusitis was often exacerbated by viral infection.A disruption of the mechanisms that regulate the activity of matrix metallopmteinases(MMPs)during viral infection was one possible mechanism responsible for the cxacerbation.The purpose of study was to achieve a better understanding of MMP expression in hasal epithelial cells after viral infection.Methotis Human nasal epithelial cells were isolated from nasal polyp specimens obtained during endoscopic endonasal surgery in chronic rhinosinusitis patients.The expression of MMP-2,MMP-9,and tissue inhibitor of mctallopmteinase (TIMP).1 mRNA in primary human nasal polyp epithelial cells after double stranded RNA(ds RNA) stimulation were investigated.Results Among the genes whose expression was evaluated,only expression of MMP-9 mRNA increased significantly after dsRNA stimulation(22.61±5.47 fold increase,Z=-2.52,P=0.012).Conclusions The significant up-regulation of MMP-9 mRNA,which Was not modulated by TIMP-1,was an additional siyrec of increased pmteolytic activity in virus-infected upper airways that might contribute to the exacerbation of chronic rhinosinusitis with nasal polyps.
