Immunological study on decellularized whole laryngeal scaffold
10.3760/cma.j.issn.1673-0860.2010.03.014
- VernacularTitle:去细胞全喉软骨支架的免疫学研究
- Author:
Rui-Na MA
1
;
Peng-Cheng CUI
;
Nan HOU
;
Jia-Sheng LUO
;
Xing WANG
Author Information
1. 第四军医大学唐都医院
- Keywords:
Larynex;
Stents;
Perfusion,regional;
Graft rejectionDOI;
10.3760/cma.j.issn. 1673-0860.2010.03.014
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2010;45(3):233-237
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the immunogenicity of the decellularized laryngeal scaffold.Methods Twelve perfused.decelluarized laryngeal scaffolds were obtained from rabbits through common carotid artery perfusion with detergents.The twelve decellularized laryngeal scaffolds and the twelve fresh larynxes were then implanted in para-laryngeal muscles of rabbits and harvested after two weeks,four weeks,twelve weeks and twenty-four weeks,respectively. Macroscopic view,histological examination and lymphocyte infihration test were performed.Results The decellularized larynxes were implanted and preserved the laryngeal extracellular matrix and laryngeal architecture.The decelluarized larynx did not show obvious immunological rejection after implanted into the para-1aryngeal muscles of the recipient rabbits.The volume of implanted larynx became smaller but retained cartilage scaffold.The larynxes in the control group presented the serious immunological rejection and the majority tissues of the larynxes were disintegrated and substituted by the fibrous connective tissues after four weeks.The peripheral tissues were damaged and necrotic at different degrees.The quantity of the lymphocyte infiltration in the control group was higher than that in the experiment group and the result had the statistical significance(P<0.01).Conclusions Perfused,decellularized technique can construct a low immune laryngeal cartilage scaffold which could be a satisfactory material for laryngeal repair.
