Immobilization of CD34 antibodies onto ethylene-vinyl acetate copolymer coatings and in vitro cell attachments.
- Author:
Min YIN
1
;
Yuan YUAN
;
Changsheng LIU
;
Jing WANG
Author Information
1. Engineering Research Center for Biomedical Materials of Ministry of Education, Institute of Biomaterials, East China University of Science and Technology, Shanghai 200237, China.
- Publication Type:Journal Article
- MeSH:
Antibodies, Immobilized;
immunology;
Antigens, CD34;
immunology;
Cell Adhesion;
drug effects;
Cells, Cultured;
Coated Materials, Biocompatible;
chemistry;
Coronary Restenosis;
prevention & control;
Endothelial Cells;
cytology;
Humans;
Peptides;
chemistry;
Polyvinyls;
chemistry;
Stents;
Umbilical Veins;
cytology
- From:
Journal of Biomedical Engineering
2008;25(5):1130-1134
- CountryChina
- Language:Chinese
-
Abstract:
Rapid re-endothelialization of implanted coronary artery stent is a new way for preventing restenosis. In this paper, adhesive polypeptide mimics of marine organism and antibodies potentially selective targeting with CD34 antigens on endothelial cells (ECs) were immobilized onto ethylene-vinyl acetate copolymer (EVA) coatings for improving the re-endothelialization process. The results showed that the attachments, growths, viabilities, as well as cell retentions of ECs on EVA were improved after modification. The immobilized polypeptide promoted the attachments of ECs, but overmuch polypeptide would restrict the attachments. With the increase of antibody concentration, the immobilized antibodies and cell attachments were improved.
