Study of polymerizing hemoglobin on cation exchange chromatography.
- Author:
Xiang WANG
1
;
Lei HUANG
;
Chenming YANG
;
Jinfeng WANG
Author Information
1. College of Pharmaceuticals and Biotechnology, Tianjin University, Tianjin 300071, China. tpuwx@sohu.com
- Publication Type:Journal Article
- MeSH:
Blood Substitutes;
chemical synthesis;
chemistry;
Chromatography, Ion Exchange;
methods;
Hemoglobins;
chemical synthesis;
chemistry;
Humans;
Pyridoxal Phosphate;
analogs & derivatives;
chemical synthesis;
chemistry
- From:
Journal of Biomedical Engineering
2008;25(5):1146-1149
- CountryChina
- Language:Chinese
-
Abstract:
Poly-hemoglobin is the active component of hemoglobin-based blood substitutes. The excess reactivity of glutaraldehyde with hemoglobin in solution leads to poly-hemoglobin of a wide molecular weight distribution and a high average molecular weight. A new polymerization method has been tested to decrease the molecular weight distribution and the average molecular weight. The poly-hemoglobin with lower degree of modification (polymerization) was found enriched on the cation exchange columns and further polymerized with glutaraldehyde. The poly-hemoglobin of narrower molecular weight distribution has been prepared in this way.
