Determination of the activity of semicarbazide-sensitive amine oxidase in the differentiated 3T3-F442A cells by enzyme-linked spectrophotometric assay.
- Author:
Sha ZHU
1
;
Yan WANG
;
Ying DU
;
Sanqiang LI
;
Xiaoyan XUAN
;
Yue TANG
Author Information
1. Department of Microbiology and Immunology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, China. zsha@zzu.edu.cn
- Publication Type:Journal Article
- MeSH:
3T3 Cells;
Adipocytes;
cytology;
enzymology;
Amine Oxidase (Copper-Containing);
metabolism;
Animals;
Cell Differentiation;
physiology;
Mice;
Spectrophotometry;
methods
- From:
Journal of Biomedical Engineering
2008;25(5):1161-1165
- CountryChina
- Language:Chinese
-
Abstract:
In order to probe into the patho-physiology semicarbazide-sensitive amine oxidase (SSAO), we determined the variation of its activity in the days of 3T3-F442A cells' differentiation into adipocytes in vitro. The differentiated 3T3-F442A cells were collected in various days and disrupted by ultrasonication. Then we studied the SSAO activity of the collected cells by oxidase-linked spectrophotometric assay. In the course of 3T3-F442A cells' differentiation, SSAO expression was shown by a curve graph. There was no SSAO detected in 3T3-F442A preadipocyte, and only a little of SSAO was detected in the cells on the 3rd day of differentiation. Since then, SSAO significantly increased and reached to a maximum level six or seven days after the cells' differentiation.
