Transfection of pEGFP-C2 in brain mediated by targeting liposome P-MMA-DOSPER.
- Author:
Shaonian TANG
1
;
Zhenhua LIU
;
Lianxu ZHAO
;
Zhihao ZOU
;
Mouxuan DU
Author Information
1. Department of Neurolog of Zhujiang Hospital Affiliated to the Southern Medical University, Guangzhou 510282, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antibodies, Monoclonal;
metabolism;
Brain;
metabolism;
Fatty Acids, Monounsaturated;
metabolism;
Female;
Gene Transfer Techniques;
Genetic Vectors;
genetics;
metabolism;
Green Fluorescent Proteins;
genetics;
metabolism;
Liposomes;
Male;
Polymethyl Methacrylate;
metabolism;
Rats;
Rats, Sprague-Dawley;
Transfection
- From:
Journal of Biomedical Engineering
2008;25(5):1170-1174
- CountryChina
- Language:Chinese
-
Abstract:
This research tried improving the specificity and efficiency of gene transfection in gene therapy and tried making the liposome a better gene transfer vector to brain by use of the monoclonal antibody (anti-Lex/SSEA-1)-mediated targeting of liposome. The derivatized monoclonal antibody was conjugated to the liposome DOSPER to form the targeting liposome P-MMA-DOSPER. Then, the pEGFP-C2 encapsulated in P-MMA-DOSPER or DOSPER was injected into the lateral ventricle of SD rats respectively, and the brains were taken for frosted slice 1, 3, 7 or 14 days later. The expression of GFP was observed under fluorescent microscope. There was a lot of expression of GFP around the lateral ventricle of rats in each group. But the indirect fluorescence antibody test showed the ratio of GFP+/nestin+ cells to nestin+ cells of every marking time point in the group of P-MMA-DOSPER was higher than the one in the group of DOSPER; the difference was found to be statistically significant (P<0.01). The results proved that the P-MMA-DOSPER can permeat the ependyma and can transfer gene into the nerve stem cells in vivo safely and effectively.
