Research progress of nanosecond pulsed electric field applied to intracellular electromanipulation.
- Author:
Chenguo YAO
1
;
Dengbin MO
;
Caixin SUN
;
Xin CHEN
;
Zheng'ai XIONG
Author Information
1. Key Lab of High Voltage Engineering and Electrical New Technology, Ministry of Education, Chongqing University, Chongqing 400044, China. yaochenguo@cqu.edu.cn
- Publication Type:Journal Article
- MeSH:
Apoptosis;
Calcium;
metabolism;
Cell Membrane;
metabolism;
Cell Nucleus;
metabolism;
Cell Physiological Phenomena;
Electromagnetic Fields;
Electroporation;
Gene Expression
- From:
Journal of Biomedical Engineering
2008;25(5):1206-1209
- CountryChina
- Language:Chinese
-
Abstract:
In recent years, many experts have done some researches on experiment and mechanism of intracellular electromanipulation (IEM) under nanosecond pulsed electric field (nsPEF). The experiment results have shown that nsPEF could not induce electroporation of cell membrane, but could induce intracellular effects such as apoptosis, calcium release, enhancement of gene expression, and fragmentation of DNA and chromosome. In order to account for the phenomenon, researchers believe that when the pulse width of the pulsed electric field is larger than the charging time of plasma membrane, the pulsed electric field mainly targets on the outer membrane of cell; and that the effect of the pulsed electric field on nucleus and nuclear membrane increases with the decrease of the pulse width. It is also believed that the effect of electroporation changes from the outer membrane to intracellular electromanipulation when the pulse width decreases to a value being smaller than the charging time of plasma membrane.
