Differentiation of human umbilical cord blood-derived mesenchymal stem cells into chondroblast and osteoblasts.
- Author:
Yanqiu YU
1
;
Haiqin REN
;
Wei YUN
;
Yunan JIN
;
Kuni LI
;
Lili DU
Author Information
1. Department of Pathophysiology, College of Basic Medical Science, China Medical University, Shenyang 110001, China. yqyu@mail.cmu.edu.cn
- Publication Type:Journal Article
- MeSH:
Cell Differentiation;
Cell Separation;
Cells, Cultured;
Chondrocytes;
cytology;
Fetal Blood;
cytology;
Humans;
Mesenchymal Stromal Cells;
cytology;
Osteoblasts;
chemistry
- From:
Journal of Biomedical Engineering
2008;25(6):1385-1389
- CountryChina
- Language:Chinese
-
Abstract:
Samples of healthy and full-term human umbilical cord blood samples were obtained asceptically. Mesenchymal stem cells (MSCs) were isolated by lymphocyte separation medium, and were characterized morphologically by fluorescence-activated cell sorting analysis. Differentiation of chondroblast and osteoblast was induced by 10 ng/ml TGF-beta, 100 ng/ml insulin and 10(-7) mol/L decaesadril, 6.25 microg/ml siderophilin, 10 mmol/L beta-sodium glycerophosphate, 50 microg/ml antiscorbic acid, respectirely; the aim was to investigate the potentiality of differentiation. Umbilical cord blood-derived MSCs were stained positive for MSCs marker CD13, CD90, CD166, CD73, CD44 and HLA-AB, but were negative for hematopoietic stem cell marker CD45, CD34 and HLA-DR. After 21 days induction, Toluidine Blue staining and von-Kossa staining were positive. Immunocytochemistry showed that Collagen II expressed in the induced cells. The results demonstrated that mesenchymal stem cells can be isolated from human umbilical cord blood and differentiated into chondroblasts and osteoblasts in vitro.
