A protein array based on quantum dots (QDs) encoded microbeads for detection of hepatitis C virus
10.3760/cma.j.issn.1003-9279.2013.01.021
- VernacularTitle:量子点编码微球蛋白芯片检测丙型肝炎病毒方法的建立
- Author:
Jun LIU
1
;
Guo-Xiang ZHANG
Author Information
1. 浙江萧山医院
- Keywords:
Quantum theory;
Microspheres;
Hepatitis C;
Antibody,viral
- From:
Chinese Journal of Experimental and Clinical Virology
2013;27(1):67-69
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a hepatitis C virus (HCV) diagnostic assay using a protein array based on the quantum dots (QDs) encoded microbeads.Method Using QDs encoded microbeads array and immunofluorescence techniques,the highly purified HCV NS3,NS4,NS5 and Core protein were respectively immobilized on the surface of encoded beads,which were used for the detection of anti-HCV antibody in serum.To evaluate the microbeads protein array,120 HCV positive and 50 HCV negative samples were tested,and compared with recombinant immunoblot assay(RIBA) results as golden standard.The sensitivity,specificity and accuracy value were calculated.Results Compared 120 positive samples detected with RIBA,the sensitivity of microbeads array is 97.50％ (117/120),the specificity is 96.0％ (48/50),and accuracy is 97.06％ [(117 + 48) / (120 + 50)],The sensitivity of microbeads protein array is similar with RIBA methods.In the 120 positive samples tested with protein array,the positive rate of antiHCV Core is 92.50％ (111/120),the positive rate of anti-HCV NS3 is 89.17％ (107/120),the positive rate of anti-HCV NS4 is 70.83％ (85/120),the positive rate of anti-HCV NS5 is 52.50％ (63/120).Conclusion The QDs encoded microbeads array demonstrated relatively high sensitivity and specificity for the diagnosis of HCV,and has clinical values.
