Evaluation of specific antibodies of blood receiver sera to Treponema pallidum by chemiluminescence immunoassay.
- Author:
Xue-chun WANG
1
;
Ling-zhi CHEN
;
Qin YU
;
Ai-hua SUN
Author Information
- Publication Type:Journal Article
- MeSH: Antibodies, Bacterial; blood; immunology; Blotting, Western; methods; Case-Control Studies; Hemagglutination Tests; methods; Humans; Immunoassay; methods; Luminescence; Luminescent Measurements; methods; Retrospective Studies; Sensitivity and Specificity; Syphilis; blood; microbiology; Treponema pallidum; immunology
- From: Chinese Journal of Experimental and Clinical Virology 2013;27(1):70-72
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThe purpose of this study was to evaluate the diagnostic performance of chemiluminescence immunoassay (CLIA ) , in comparison with that of the following currently used treponemal tests: hemagglutination test (TPHA), and Western Blot (WB).
METHODSFirst, a retrospective study was performed with a panel of 18 494 blood receiver sera by CLIA and TPHA, the specific antibody against T. pallidum in 177 were positive sera by CLIA and/or TPHA, 81 clinical and serologically characterized syphilitic sera, 55 sera obtained from subjects with potentially interfering diseases, and 250 healthy sera were negative were detected by CLIA, TPHA and WB.
RESULTSThe results of WB as the gold standard, the sensitivity of CLIA (98. 4%) was significantly higher than that of TPHA (94. 4%) (Chi2 = 5.76,P <0. 05), the specificity of CLIA (100%) was similar to that of TPHA (99.7%) (Chi2 =1. 0, P >0. 05).
CONCLUSIONCLIA is characterized with higher sensitivity and specificity, It is suitable for screening Syphilis in clinical laboratory.
