Evaluation of specific antibodies of blood receiver sera to Treponema pallidum by chemiluminescence immunoassay

VernacularTitle:化学发光法检测受血者梅毒螺旋体特异性抗体的方法学评价
Author: Xue-Chun WANG ¹ ; Ling-Zhi CHEN ; Qin YU ; Ai-Hua SUN
Author Information

1. 314001,浙江嘉兴市第一医院输血科
Keywords: Treponema pallidum; Antibody/syphilis; Chemiluminescence
From: Chinese Journal of Experimental and Clinical Virology 2013;27(1):70-72
CountryChina
Language:Chinese
Abstract: Objective The purpose of this study was to evaluate the diagnostic performance of chemiluminescence immunoassay (CLIA),in comparison with that of the following currently used treponemal tests:hemagglutination test (TPHA),and Western Blot (WB).Methods First,a retrospective study was performed with a panel of 18 494 blood receiver sera by CLIA and TPHA,the specific antibody against T.pallidum in 177 were positive sera by CLIA and/or TPHA,81 clinical and serologically characterized syphilitic sera,55 sera obtained from subjects with potentially interfering diseases,and 250 healthy sera were negative were detected by CLIA,TPHA and WB.Results The results of WB as the gold standard,the sensitivity of CLIA (98.4％) was significantly higher than that of TPHA (94.4％) (x2 =5.76,P ＜ 0.05),the specificity of CLIA (100％) was similar to that of TPHA (99.7％)(x2 =1.0,P ＞ 0.05).Conclusion CLIA is characterized with higher sensitivity and specificity,It is suitable for screening Syphilis in clinical laboratory.