Virus detection in bronchoalveolar lavage fluid of 122 children with severe pneumonia.
- Author:
Le-Yun XIE
1
;
Li-Li ZHONG
;
Bing ZHANG
;
Zhao-Jun DUAN
;
Zhi-Ping XIE
;
Han-Chun GAO
;
Qian-Qian CHEN
;
Zhong-Hua DENG
;
Lin LIN
;
Xiao-Qian GAO
;
Ni-Guang XIAO
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviruses, Human; isolation & purification; Adolescent; Bronchoalveolar Lavage Fluid; virology; Child; Child, Preschool; Female; Humans; Infant; Male; Pneumonia; virology; Reverse Transcriptase Polymerase Chain Reaction; Seasons; Viruses; isolation & purification
- From: Chinese Journal of Experimental and Clinical Virology 2013;27(2):95-97
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the prevalence of viral pathogen in children with severe pneumonia in Hunan.
METHODBronchoalveolar lavage fluid [BALF] were collected from 122 hospitalized children with severe pneumonia in People's Hospital of Hunan province from January 2011 to December 2011. Nested- or reverse transcription Polymerase chain reaction (PCR or RT-PCR) was used to screen Adenovirus (ADV), Human Bocavirus (HBoV), Parainfluenzaviruses1-4 (PIV1-4), Human Respiratory Syneytial virus (RSV), Influenza virus A (IFVA), Influenza virus B (IFVB), Human Rhinovirus(HRV), Human Metapneumovirus (HMPV), human coronaviruses NL63 and HKU1 (HCoV-NL63, HCoV- HKU1).
RESULTSAmong the 122 bronchoalveolar lavage fluid, viral agents were detected in 60 samples(49.1%), among which ADV (40.98%) was the most common virus, followed by RSV (7.37%) and HBoV (7.37%). Two viruses were detected in 21 individual (35%) samples, of which 20 were dual positive for ADV (40%).
CONCLUSIONADV is the most frequently detected viral etiology of severe pneumonia in children in Hunan during this year. And its Coinfection with other respiratory viruses was common.
