Detection of human parvovirus B19, human bocavirus and human parvovirus 4 infections in blood samples among 95 patients with liver disease in Nanjing by nested PCR.
- Author:
Rui TONG
1
;
Wei-Min ZHOU
;
Xi-Jun LIU
;
Yue WANG
;
Yong-Liang LOU
;
Wen-Jie TAN
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Child; Coinfection; virology; Female; Human bocavirus; isolation & purification; Humans; Liver Diseases; virology; Male; Middle Aged; Parvovirus; isolation & purification; Parvovirus B19, Human; isolation & purification; Polymerase Chain Reaction; Viremia; virology
- From: Chinese Journal of Experimental and Clinical Virology 2013;27(2):135-137
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the infection of human parvovirus B19, human bocavirus (HBoV) and human parvovirus 4 (PARV4) in blood samples among patients with liver disease in Nanjing by molecular detection.
METHODSNested PCR assays were designed and validated to detect B19, HBoV and PARV4, respectively. The assays were used to screen three parvoviruses in blood samples from 95 patients with different liver disease in Nanjing. The parvovirus infection was analyzed statistically.
RESULTSThe detection limits were 10 copies of genomic DNA equivalents per reaction for each assays and the good specificity were observed. The frequency of B19 and HBoV were 2/95 (2.1%) and 9/95 (9.5%) in blood samples respectively. No PARV4 was detected. HBoV was detected in 3/5 patients with drug-induced hepatitis.
CONCLUSIONBoth B19 and HBoV infection were detected in blood from patients with liver disease.
