Preparation and characterization the polyclonal antibody of the nonstructural protein of human highly pathogenic H5N1 avian influenza viruses.
- Author:
Pei-Yu JIANG
1
;
Hui-Lian HUANG
;
Hong-Chang ZHOU
;
Bo-Ying XU
;
Fu-Ping GU
;
Li-Shan MIN
;
Jing ZHONG
;
Li-Cheng DAI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies, Viral; biosynthesis; immunology; Escherichia coli; genetics; Influenza A Virus, H5N1 Subtype; immunology; Rabbits; Recombinant Fusion Proteins; immunology; isolation & purification; Viral Nonstructural Proteins; genetics; immunology
- From: Chinese Journal of Experimental and Clinical Virology 2013;27(2):138-140
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEOf this study was to prepare high sensitivity and high specificity of highly pathogenic H5N1 subtype avian influenza virus NS1 protein antibody and a preliminary assessment of its potency.
METHODSConstruct pET-28a (+) recombinant vector containing the H5N1 subtype of avian influenza virus NS1 sequences of E. coli BL21 (DE3), induced expression of NS1 protein, NS1 recombinant protein was obtained by Ni-NTA column purified by affinity chromatography, and SDS-PAGE and Western Blot analysis. Purified protein antigen to immunize New Zealand white rabbits, obtained rabbit anti-NS1 serum, affinity-purified polyclonal antibodies. Using ELISA and Western Blot analysis of purified antibody titer and specificity.
RESULTSNS1 fusion protein was highly expressed in a purity of greater than 90%, with the fusion protein was used to immunize New Zealand white rabbits anti-NS1 polyclonal antibody titer of 1:80 000, and specific recognition of the H5N1 subtype of avian influenza virus NS1 protein.
CONCLUSIONSNS1 polyclonal antibodies to NS1 recombinant protein purified antigen, with better potency and specificity, and to prepare the conditions for the development of the H5N1 subtype of avian influenza virus detection kit.
