Subcellular localization of severe fever with thrombocytopenia syndrome virus in macrophages.
- Author:
Ying HAN
1
;
Yi-Xiang LI
;
Cong JIN
;
Chuan LI
;
Jing QU
;
Fu-Shun ZHANG
;
Quan-Fu ZHANG
;
Mi-Fang LIANG
;
Pei-Hong QIU
Author Information
- Publication Type:Journal Article
- MeSH: Bunyaviridae; isolation & purification; Cell Line, Tumor; Endoplasmic Reticulum; virology; Fever; virology; Golgi Apparatus; virology; Humans; Macrophages; virology; Thrombocytopenia; virology
- From: Chinese Journal of Experimental and Clinical Virology 2013;27(3):161-163
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the subcellular localization of severe fever with thrombocytopenia syndrome virus (SFTSV) in macrophages and understand the replication and assembly mechanism of SFTSV in host cells.
METHODSUsing two types of human macrophage cell lines THP-1 and U937, the study analyzed the intracellular colocalization of SFTSV with Golgi apparatus and endoplasmic reticulum by immunefluorescence staining and confocal microscopy.
RESULTSSFTSV infected macrophage cell lines THP-1 and U937. Immunofluorescence staining showed that the SFTSV nuclear protein colocalized with Golgi apparatus and closely surrounded by endoplasmic reticulum in the perinuclear region.
CONCLUSIONThe results suggested that Golgi complex and endoplasmic reticulum are probably the sites for formation and maturation of SFTSV viral particles.
