Genetic characterization of G II.12 norovirus in Beijing from 2008 to 2009.
- Author:
Ke-Na CHEN
1
;
Geng TIAN
;
Miao JIN
;
Hui-Ying LI
;
Quan-Rui LI
;
Li-Hong KANG
;
Cui-Hong ZHANG
;
Xiang-Yu KONG
;
Ji-In GAO
;
Zhao-Jun DUAN
Author Information
- Publication Type:Journal Article
- MeSH: China; Norovirus; classification; genetics; Phylogeny; Recombination, Genetic; Time Factors
- From: Chinese Journal of Experimental and Clinical Virology 2013;27(3):184-186
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo reveal the genetic characteristics of GII.12 Norovirus strains isolating from stool samples of adults with diarrhea in Beijing during 2008-2009.
METHODSRdRp, ORF2, ORF3 and ORF1/ORF2 overlap region were respectively amplified by primers using RT-PCR. The products were purified, cloned, sequenced and then aligned, phylogenetic and recombinant analyzed by softwares of DNAStar, MEGA and SimPlot.
RESULTSAccording to the phylogenetic analysis, 11 strains belonged to G II.g in the RdRp region,while GII.12 in the ORF2 and ORF3. SimPlot analysis further confirmed the 11 strains were recombinant strains ( G II.g [RdRp]/G II.12 [capsid]).
CONCLUSIONG II.12 Norovirus prevailing in Beijing and other regions of the world belonged to the same strain, and we identified the genetic characteristics of G II.12 Norovirus in Beijing.
