Interleukin-32 expression in serum of patients with HBV-related liver failure and its significance.
- Author:
Gui-Long ZHUANG
1
;
Guo-Hang LI
1
;
Zhi-Jun QU
1
;
Jian-Yu KUANG
1
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Alanine Transaminase; blood; Aspartate Aminotransferases; blood; Female; Hepatitis B virus; isolation & purification; physiology; Hepatitis B, Chronic; blood; enzymology; genetics; virology; Humans; Interleukins; blood; genetics; Liver Failure; blood; enzymology; genetics; virology; Male; Middle Aged; Viral Load; Young Adult
- From: Chinese Journal of Experimental and Clinical Virology 2013;27(4):247-249
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression level of IL-32 in serum and its correlation with serum biochemical indices of liver function test and HBV DNA load in patients with HBV-related liver failure.
METHODSFifty-five patients with HBV-related liver failure (severe hepatitis group) and twenty normal cases (control group) were enrolled in the study. Total RNA in PBMCs was extracted by using TRIzol. IL-32 mRNA level was assayed by using Real-time PCR. IL-32 protein level in serum was detected by ELSIA method. The correlation between IL-32 and ALT, AST, TBIL, HBV DNA load was analyzed using pearson's correlation analysis, respectively.
RESULTSSerum IL-32 expression level in severe hepatitis group was higher than that of control group. Moreover, the difference between them was statistically significant (P < 0.05). Serum IL-32 level was positively correlated with serum ALT, AST, TBIL, respectively (P < 0.05), but was not correlated with HBV DNA load (P > 0.05).
CONCLUSIONSerum IL-32 expression level was increased in patients with HBV-related liver failure and was associated with the severity of inflammation. We, therefore, believe that IL-32 might be involved in the pathogenesis of HBV-related liver failure.
