Interleukin-32 expression in serum of patients with HBV-related liver failure and its significance
10.3760/cma.j.issn.1003-9279.2013.04.003
- VernacularTitle:乙肝相关性肝衰竭患者外周血中IL-32表达水平及意义
- Author:
Gui-Long ZHUANG
1
;
Guo-Hang LI
;
Zhi-Jun QU
;
Jian-Yu KUANG
Author Information
1. 深圳市龙岗中心医院感染科
- Keywords:
Interleukin-32;
Liver failure;
Hepatitis B
- From:
Chinese Journal of Experimental and Clinical Virology
2013;27(4):247-249
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression level of IL-32 in serum and its correlation with serum biochemical indices of liver function test and HBV DNA load in patients with HBV-related liver failure.Methods Fifty-five patients with HBV-related liver failure (severe hepatitis group) and twenty normal cases (control group) were enrolled in the study.Total RNA in PBMCs was extracted by using TRIzol.IL-32 mRNA level was assayed by using Real-time PCR.IL-32 protein level in serum was detected by ELSIA method.The correlation between IL-32 and ALT,AST,TBIL,HBV DNA load was analyzed using pearson's correlation analysis,respectively.Results Serum IL-32 expression level in severe hepatitis group was higher than that of control group.Moreover,the difference between them was statistically significant (P ＜0.05).Serum IL-32 level was positively correlated with serum ALT,AST,TBIL,respectively (P ＜ 0.05),but was not correlated with HBV DNA load (P ＞ 0.05).Conclusion Serum IL-32 expression level was increased in patients with HBV-related liver failure and was associated with the severity of inflammation.We,therefore,believe that IL-32 might be involved in the pathogenesis of HBV-related liver failure.
