The effects of recombinant human beta-defensin-3 on expression of interleukin-17A and interleukin22 in BEAS-2B cell
10.3760/cma.j.issn.1003-9279.2013.04.007
- VernacularTitle:重组人β防御素3对BEAS-2B细胞IL-17A和IL-22表达的影响
- Author:
Bing-Ya GUO
1
;
Guang-Cheng XIE
;
Zhao-Jun DUAN
;
Li-Jun XIA
Author Information
1. 山西医科大学第一医院耳鼻咽喉头颈外科
- Keywords:
Beta-defensins;
Receptor-Toll;
Interleukin-17A;
Interleukin-22
- From:
Chinese Journal of Experimental and Clinical Virology
2013;27(4):260-262
- CountryChina
- Language:Chinese
-
Abstract:
Objective To research the effects of recombinant human beta-defensin-3 (hBD-3) on expression of interleukin-17A (IL-17A) and interleukin-22 (IL-22) in BEAS-2B cell.Methods The BEAS-2B cells were stimulated with different concentrations of hBD-3 for 6 hours and 24 hours,respectively.Toll-like receptor 2 (TLR2),IL-17A and IL-22 mRNA expression levels were determined by real-time PCR,and the expression levels of IL-17A and IL-22 protein were examined by enzyme linked immune-sorbent assay.Results TLR2 mRNA in BEAS-2B cells were significantly increased in a concentration-and time-dependent manner after stimulating by hBD-3 for 24 hours compared to 6 hours.The IL-17A has significantly increased in mRNA and protein levels stimulated 24 hours in a concentration of 100ng/ml,however,IL-17A mRNA expression has increased while protein didn't change stimulated 6 hours in a concentration of 50 ng/ml.The IL-22 mRNA and protein expression reached peak levels after stimulating in a concentration of 50 ng/ml of hBD-3 while IL-22 expression declined in mRNA and protein levels as the concentration of hBD-3 increased.Conclusions Recombinant hBD-3 can up-regulated the expression of TLR2,IL-17A and IL-22,lower concentration of hBD-3 mainly increased the expression of IL-22 while higher concentration of hBD-3 mainly increased the expression of IL-17A.These results show that different concentrations of hBD-3 maybe activate different transcription factors which was mediated by TLR2,initiating host immune response.
