Construction of recombinant CHO cell strain for high expression of HBsAg.
- Author:
Yao YI
1
;
Min-Zhuo GUO
1
;
Rui-Guang TIAN
1
;
Qiu-Dong SU
1
;
Xue-Xin LU
1
;
Feng QIU
1
;
Wen-Ting ZHOU
1
;
Zhi-Yuan JIA
1
;
Sheng-Li BI
1
Author Information
- Publication Type:Journal Article
- MeSH: Animals; CHO Cells; Cricetinae; Cricetulus; Gene Expression; Hepatitis B Surface Antigens; genetics; immunology; Transfection
- From: Chinese Journal of Experimental and Clinical Virology 2013;27(4):289-291
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo overexpress hepatitis B virus S gene in CHO cells cultured in serum-free media.
METHODPlasmid was constructed by cloning of HBV S gene and then it was transfected into CHO cells. After cell screen, the positive clones were identified and isolated into a serum-free media followed by the serological and morphological characterization of the expression product.
RESULTCHO cell strains which can express HBsAg efficiently and stably were obtained. Spherical and filamentous HBsAg could be detected under electronic microscope. The titer of the expression product was up to 1:5000.
CONCLUSIONSerum-free media cultured CHO cell strain for overexpression of HBsAg was successfully constructed and the expression product was high antigenic.
