Establishment of a detection method for hepatitis B virus large surface protein (HBV-lP).
- Author:
Jing ZHAO
1
;
Hong-Bin MA
1
;
Jun HOU
1
;
Jing-Xia GUO
1
;
Jun XU
1
;
Yong-Ji SONG
1
;
Lin CHEN
1
;
Ai-Xia LIU
1
;
Jia LIU
1
;
Hong-Shan WEI
1
;
Bo-An LI
1
Author Information
- Publication Type:Journal Article
- MeSH: Enzyme-Linked Immunosorbent Assay; methods; Hepatitis B; blood; diagnosis; virology; Hepatitis B Surface Antigens; blood; Hepatitis B virus; isolation & purification; metabolism; Humans
- From: Chinese Journal of Experimental and Clinical Virology 2013;27(4):292-294
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish enzyme-linked immunosorbent assay (ELISA) for detection of hepatitis B virus large surface protein(HBV-LP) in serum.
METHODSA sandwich reaction was preformed with horseradish peroxidase labeled monoclonal antibody of HBV-LP as the catalytic enzyme. Several reactions liquid's concentration and reaction conditions were optimized. The method was evaluated in all aspects such as sensitivity, specificity, stability and so on.
RESULTSThe detection limit was 5 ng/ml. Interassay and intra-assay RSD were both less than 10%. After stored at 4 degrees C and 37 degrees C for 3, 5, 7 days, the analysis showed correlation coefficient higher than 0.98 and RSD lower than 10%.
CONCLUSIONEstablished ELISA for determination of serum HBV-LP has high sensitivity and repeatability. Enzyme-linked immunosorbent assay;
