Comparison of two kinds of enzyme-linked immunosorbnent assay for detection of anti-hepatitis C.
- Author:
Long-You ZHAO
1
;
Yong-Ping JI
1
;
De-Bin WANG
1
;
Jie ZHUANG
1
;
Bin ZHOU
1
Author Information
- Publication Type:Journal Article
- MeSH: Enzyme-Linked Immunosorbent Assay; methods; Hepatitis C; blood; diagnosis; Hepatitis C Antibodies; blood; Humans; Reagent Kits, Diagnostic; Sensitivity and Specificity
- From: Chinese Journal of Experimental and Clinical Virology 2013;27(4):304-306
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the difference of sensitivity and specificity on the results of the same samples determined by indirect ELISA and sandwich ELISA.
METHODSA 51 anti-HCV positive serum samples obtained from donors were screened by two indirect ELISA kits initially, and then were detected using one sandwich ELISA kit, and were confirmed by recombinent immunoblot assay (RIBA) and HCV-RNA.
RESULTSTo compared with HCV-RNA, false positive rate of two kinds of indirect ELISA and one sandwich ELISA was 40.9%, 59.1% and 2.2% respectively. The positive rates of them were 100%, but the analytical sensitivity of sandwich ELISA was more than indirect ELISA 2 to 6 times.
CONCLUSIONSThe sensitivity and specificity of sandwich ELISA was significantly better than those of indirect ELISA.
