Comparison of two kinds of enzyme-linked immunosorbnent assay for detection of anti-hepatitis C virus

VernacularTitle:两种酶联免疫法检测丙型肝炎病毒抗体结果分析
Author: Long-You ZHAO ¹ ; Yong-Ping JI ; De-Bin WANG ; Jie ZHUANG ; Bin ZHOU
Author Information

1. 323000,浙江省丽水市中心血站
Keywords: Hepatitis C antibodies; Enzyme-Linked immunosorbent assay; Sensitivity and specificity
From: Chinese Journal of Experimental and Clinical Virology 2013;27(4):304-306
CountryChina
Language:Chinese
Abstract: Objective To compare the difference of sensitivity and specificity on the results of the same samples determined by indirect ELISA and sandwich ELISA.Methods A 51 anti-HCV positive serum samples obtained from donors were screened by two indirect ELISA kits initially,and then were detected using one sandwich ELISA kit,and were confirmed by recombinent immunoblot assay (RIBA) and HCV-RNA.Results To compared with HCV-RNA,false positive rate of two kinds of indirect ELISA and one sandwich ELISA was 40.9％,59.1％ and 2.2％ respectively.The positive rates of them were 100％,but the analytical sensitivity of sandwich ELISA was more than indirect ELISA 2 to 6 times.Conclusions The sensitivity and specificity of sandwich ELISA was significantly better than those of indirect ELISA.