Protective effects of rhein on hepatic progression in HBV-transgenic mice with nonalcoholic steatohepatitis induced by a high-fat diet
10.3760/cma.j.issn.1003-9279.2013.05.004
- VernacularTitle:大黄酸对合并非酒精性脂肪性肝炎的HBV转基因小鼠肝病恶化的防治作用
- Keywords:
Fatty liver;
Hepatitis B virus;
Transgenic mouse
- From:
Chinese Journal of Experimental and Clinical Virology
2013;27(5):328-331
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the beneficial effects of Rhein (RH) on hepatic progression in hepatitis B virus (HBV)-transgenic mice with nonalcoholic steatohepatitis induced by a high-fat (HF) diet.Methods A mice model of HBV chronic infection concomitant with liver steatosis was induced by a HF diet in 4-week old HBV-transgenic mice for 16 weeks (n =130).Thereafter,the mice were divided randomly into control group (back to normal chow),model group (continuing HF diet),RH group [continuing HF diet and administering with 120 mg/(kg· d) RH by gavage] and Essentiale group [continuing HF diet and administering with 69.2 mg/(kg·d) Essentiale by gavage] with 30 mice in each,and were sacrificed at the end of 24-week and 48-week respectively.Serum levels of alanine aminotransferase (ALT),aspartate aminotransferase (AST),total cholesterol (TC),triglyceride (TG) and fasting plasma glucose (FPG) were measured by an automatic biochemical analyzer,and serum HBV-DNA was determined with qPCR.Hepatic histology was evaluated by HE staining with a light microscope.Results ①An histological change composed of steatosis,lymphocytes intralobular infiltration and ballooning was observed after 48 weeks feeding of HF diet,in part mimicking that of NASH patients as evidenced by a NAFLD activity score (NAS) of 3.58 ± 1.44 points.②Histologically,the NAS of model group was higher than that of control group at both time points.RH failed to lessen NAS whereas Essentiale improved the NAS at 48-week.③Serum levels of TC,TG and FPG were significantly different between 4 groups at 24-week,with a comparable low value in both RH and Essentiale group.A similar change was evident at 48-week.④In terms of HBV viral load,a significantly lower level in Essentiale group than the others was observed at both time points.Conclusion HF diet feeding is able to induce a mouse model of HBV chronic infection concomitant with NASH.RH is effective in alleviating the glucose and lipid metabolism but ineffective in improving the hepatic histology in this model,in contrast,backing to normal chow achieved a better effect in this aspect.
