Expression of TGF-β1/Smad protein in rat liver fibrosis model and the role of IFN-γ
10.3760/cma.j.issn.1003-9279.2013.05.007
- VernacularTitle:实验性肝纤维化TGF-β1/Smad的表达及γ-干扰素对其的作用
- Author:
Zhao-Bin CAI
1
;
Xiao-Qing FU
;
Shou-Rong LIU
;
Jian-Chun GUO
;
Jian-Feng BAO
Author Information
1. 310023,杭州市西溪医院(原杭州市第六人民医院)
- Keywords:
Liver cirrhosis;
Receptor/transforming growth Factorβ;
Protein smad;
Interferin type Ⅱ
- From:
Chinese Journal of Experimental and Clinical Virology
2013;27(5):340-343
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the impact of IFN-γ on liver fibrosis and its possible mechanism.Methods Thirty healthy male SD rats were randomly divided into two groups:fibrosis model group,IFN-γ treatment group.Experimental liver fibrosis was induced by subcutaneous injection of CCl4.After 12-week-treatment,serum hyalurnic acid and TGF-β1 was examined,histopathological changes and degrees of fibrosis were observed by optical microscopy.Meanwhile,the expression of TGF-β1,TβR-Ⅰ and Smad2/3 proteins was detected by immunohistochemistry and quantified by using computerized image analysis.Results ① Pathological observation of hepatic specimens:histological examination showed that there were significant difference between normal group and fibrosis model group by comparing with the degrees of inflammation and fibrosis(P ＜ 0.05).And the difference between fibrosis model group and IFN-γ treatment group was significant(P ＜ 0.05).②Changes of the hepatic fibrosis index (serum HA and TGF-β1):the levels of serum HA,TGF-[β1 in fibrosis model group were higher than IFN-γ treatment groups(P ＜ 0.05).③Changes of gene protein levels about TGF-β/Smad:the expressions of TGF-β1,TβR-Ⅰ and Smad2/3 in rat hepatic tissue were detected with immunohistochemistry techniques.The expressions of the three items in model group were higher than normal group(P ＜0.01).The difference between model group and IFN-γ treatment group was significant (P ＜ 0.05) ; Conclusion IFN-γ treatment group had significant results on treating experimental hepatic fibrosis.By the way of inhibiting expressions of TGF-β1,TβR-Ⅰ,Smad2/3,IFN-γ treatment group exerted its anti-fibrosis effect.
