Distribution of genotypes in ESBLs producing E.coli strains isolated from posthepatitic cirrhosis' patients with bloodstream infection
10.3760/cma.j.issn.1003-9279.2013.05.009
- VernacularTitle:病毒性肝炎后肝硬化住院患者血液感染大肠埃希菌超广谱β-内酰胺酶基因型调查
- Author:
Tong-Sheng GUO
1
;
En-Bo CUI
;
Chun-Mei BAO
;
Ju-Ling ZHANG
;
Fen QU
;
Yuan-Li MAO
;
Yu-Long CONG
Author Information
1. 解放军军医学院
- Keywords:
Liver cirrhosis;
Blood;
Escherichia coli;
Penicillinase
- From:
Chinese Journal of Experimental and Clinical Virology
2013;27(5):348-350
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the genotype distribution of extended-spectrum β-lactamases (ESBLs) in ESBLs-producing Escherichia coli (E.coli) isolates from posthepatitic cirrhosis' patients with bloodstream infection.Methods E.coli were isolated in bloodstream from patients with posthepatitic cirrhosis between January and December in 2011.The strains were identified by VITEK-Ⅱ.The antibiol susceptibility tests were performed with K-B method.β-lactamases genes were detected multi-PCR,PCR,sequence and blast.Results A total of 79 non-duplicate clinical isolates of E coli were consecutively collected from liver cirrhosis' patients with bloodstream infection.There were 20 isolates produced TEM-1 type β-lactamases and 1 isolate produced SHV-1 typeβ-lactamases.40 clinical isolates were detected to produce CTX-M type ESBLs,there were 20 CTX-M-1 group and 26 CTX-M-9 group,including 6 stains habouring both CTX-M-1 and CTX-M-9 group.Eight CTX-M genotypes were confirmed by sequencing of the PCR products,including CTX-M-3,CTX-M-14,CTX-M-15,CTX-M-24,CTX-M-28,CTX-M-31,CTX-M-65 and CTX-M-79.Conclusion CTX-M genotype ESBLs was the most popular extended-spectrum β-lactamases in E.coli isolated from liver cirrhosis' patients with bloodstream infection.The CTX-M-14 is the dominant epidemic type.
