Relationship between serum HBV DNA level and follicular helper T lymphocyte in patients with chronic hepatitis B and its significance.
- Author:
Juan-Hua WANG
;
Xi-Bing GU
;
Yin-Fang ZHU
;
Zhong HUA
;
Dong WANG
;
Xiao-Juan YANG
;
Yue-Qin XU
;
Zhong-Hua LU
- Publication Type:Journal Article
- MeSH: Adult; CD4 Lymphocyte Count; DNA, Viral; blood; genetics; Female; HLA-A2 Antigen; immunology; Hepatitis B virus; genetics; isolation & purification; Hepatitis B, Chronic; blood; immunology; virology; Humans; Interleukins; immunology; Male; T-Lymphocytes, Helper-Inducer; cytology
- From: Chinese Journal of Experimental and Clinical Virology 2013;27(5):351-353
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore relationship between HBV DNA level and peripheral blood follicular helper T lymphocyte (Tfh) in patients with chronic hepatitis B (CHB) and its significance.
METHODSHBV DNA levels of 179 cases of CHB patients with positive HBV DNA, positive HBeAg and positive human leukocyte antigen(HLA)-A2 were tested with real time fluorescent quantitative PCR. Tfh and HBV specific CTL were tested with flow cytometry. IL-21 was also tested. 179 cases of CHB patients were divided into group A and group B based on HBV DNA levels, 86 cases in group A, HBV DNA levels were 10(4)-10(5) copies/ml, 93 cases in group B, HBV DNA levels were 10(6)-10(7) copies/ml. Above testing indexes of the two groups were compared.
RESULTSHBV DNA levels of group A were (4.85 +/- 0.37) log10 copies/ml, HBV DNA levels of group B were (6.83 +/- 0.31 ) log10 copies/ml, t = 27.31, P < 0. 001; Tfh of group A was (5.96 +/- 1.59)%, higher than that of group B (3.71 +/- 2.15)%, t = 4.92, P < 0.01; IL-21 of group A was (42.61 +/- 15.11)ng/L, higher than that of group B (14.91 +/- 3.15) ng/L, t = 8.62, P < 0.01; HBV specific CTL of group A was (0.36 +/- 0.08)%, higher than that of group B (0.18 +/- 0.06)%, t = 19.99, P < 0.001.
CONCLUSIONSerum HBV DNA level of CHB patients is related to the level of peripheral blood Tfh level: patients with low HBV DNA level have high Tfh level, high IL-21 level and high HBV specific CTL level. Patients with high HBV DNA level have low Tfh level, low IL-21 level and low HBV specific CTL level. The mechanism of baseline HBV DNA level affecting anti-viral therapy may be related to Tfh level.