Relationship between serum HBV DNA level and follicular helper T lymphocyte in patients with chronic hepatitis B and its significance
10.3760/cma.j.issn.1003-9279.2013.05.010
- VernacularTitle:慢性乙型肝炎患者血清HBV DNA水平与滤泡辅助性T淋巴细胞的关系和意义
- Keywords:
Hepatitis B,chronic;
DNA;
T lymphocytes;
Interleukin-21;
T lymphocytes,cytotoxic
- From:
Chinese Journal of Experimental and Clinical Virology
2013;27(5):351-353
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore relationship between HBV DNA level and peripheral blood follicular helper T lymphocyte (Tfh) in patients with chronic hepatitis B (CHB) and its significance.Methods HBV DNA levels of 179 cases of CHB patients with positive HBV DNA,positive HBeAg and positive human leukocyte antigen (HLA)-A2 were tested with real time fluorescent quantitative PCR.Tfh and HBV specific CTL were tested with flow cytometry.IL-21 was also tested.179 cases of CHB patients were divided into group A and group B based on HBV DNA levels,86 cases in group A,HBV DNA levels were 104-105copies/ml,93 cases in group B,HBV DNA levels were 106-107 copies/ml.Above testing indexes of the two groups were compared.Results HBV DNA levels of group A were (4.85 ± 0.37) log10 copies/ml,HBV DNA levels of group B were(6.83 ±0.31) log10copies/ml,t =27.31,P ＜0.001 ; Tfh of group A was (5.96±l.59)％,higherthanthatofgroupB(3.71 ±2.15)％,t=4.92,P＜0.01; IL-21 of group A was (42.61 ±15.11)ng/L,higher than that of group B(14.91 ±3.15) ng/L,t =8.62,P ＜0.01; HBV specific CTL of group A was(0.36 ± 0.08)％,higher than that of group B (0.18 ± 0.06)％,t =19.99,P ＜ 0.001.Conclusion Serum HBV DNA level of CHB patients is related to the level of peripheral blood Tfh level:patients with low HBV DNA level have high Tfh level,high IL-21 level and high HBV specific CTL level.Patients with high HBV DNA level have low Tfh level,low IL-21 level and low HBV specific CTL level.The mechanism of baseline HBV DNA level affecting anti-viral therapy may be related to Tfh level.
