Formation and identification of virus-like particles of poliovirus type I.
- Author:
Xiao-Wen WANG
;
Wang SHENG
;
Yi ZENG
- Publication Type:Journal Article
- MeSH: Female; Gene Expression; Humans; Male; Poliomyelitis; prevention & control; virology; Poliovirus; genetics; metabolism; Poliovirus Vaccines; genetics; metabolism; Saccharomyces cerevisiae; genetics; metabolism; Viral Proteins; genetics; metabolism; Virion; genetics; metabolism
- From: Chinese Journal of Experimental and Clinical Virology 2013;27(5):373-375
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method to produce virus-like particles (VLP) of poliovirus type I in Saccharomy cescerevisiae to develop potential novel recombinant vaccine against poliovirus type 1.
METHODSThe genes of P1 and 3CD of poliovirus type I were optimized, synthesized and inserted into expression vector, which was further transfected into Saccharomy cescerevisiae. The extracts of yeast cells were purified by CsCl density gradient centrifugation after induction and cell lysis.
RESULTSElectrophoresis and sequencing analyses showed that the genes P1 and 3CD of poliovirus type I were successfully inserted into expression vector and encode a protein whose amino acid sequences were identical with wide-type genes of poliovirus type I. Electronic microscopy analysis showed that the VLPs of poliovirus type I could be efficiently formed in Saccharomy cescerevisiae.
CONCLUSIONThe VLPs of poliovirus type I could be efficiently produced by co-expression of P1 and 3CD genes in Saccharomy cescerevisiae.