Formation and identification of virus-like particles of poliovirus type Ⅰ

VernacularTitle:脊髓灰质炎Ⅰ型病毒病毒样颗粒的制备及鉴定
Author: Xiao-Wen WANG ¹ ; Wang SHENG ; Yi ZENG
Author Information

1. 北京工业大学生命科学与生物工程学院病毒药理室
Keywords: Poliovirus; Virus-like particle; Vaccine
From: Chinese Journal of Experimental and Clinical Virology 2013;27(5):373-375
CountryChina
Language:Chinese
Abstract: Objective To establish a method to produce virus-like particles (VLP) of poliovirus type Ⅰ in Saccharomy cescerevisiae to develop potential novel recombinant vaccine against poliovirus type 1.Methods The genes of P1 and 3CD of poliovirus type Ⅰ were optimized,synthesized and inserted into expression vector,which was further transfected into Saccharomy cescerevisiae.The extracts of yeast cells were purified by CsCl density gradient centrifugation after induction and cell lysis.Results Electrophoresis and sequencing analyses showed that the genes P1 and 3CD of poliovirus type Ⅰ were successfully inserted into expression vector and encode a protein whose amino acid sequences were identical with wide-type genes of poliovirus type Ⅰ.Electronic microscopy analysis showed that the VLPs of poliovirus type Ⅰ could be efficiently formed in Saccharomy cescerevisiae.Conclusion The VLPs of poliovirus type Ⅰ could be efficiently produced by co-expression of P1 and 3CD genes in Saccharomy cescerevisiae.