Establishment of localization ultrathin section for cytopathic cells

VernacularTitle:病变细胞定位超薄切片方法的建立
Author: Jing-Dong SONG ¹ ; Jian-Guo QU ; Tao HONG
Author Information

1. 中国疾病预防控制中心病毒病预防控制所传染病预防控制国家重点实验室
Keywords: Microscopy,electron; Cell line/ultrastructure
From: Chinese Journal of Experimental and Clinical Virology 2013;27(5):376-378
CountryChina
Language:Chinese
Abstract: Objective To establish a localization ultrathin section method through which target cytopathic cells could be sectioned in situ.Methods Lab-Tek Chamber slideTM system (177402) was selected as resin embedding mould.Cells infected with Human adenovirus type 5 (AdS) or A/HN/SWL3/2009(H1N1) influenza virus were embedded in situ as models.Target cytopathic cells were exposed by trimming,sectioned and observed under transmission electron microscope(TEM).Results Target cells could be sectioned in situ and virus particles could be found easily on sections.Conclusion A localization ultrathin sectioning method was established and this technique could be applied in virus detection in cytopathic cells to improve TEM detection efficiency.