Rapidly detect and distinguish between Norovirus G Ⅰ and G Ⅱ type with a pair of primers

VernacularTitle:一对引物快速检测并区分诺如病毒GⅠ型和GⅡ型的探讨
Author: Jian-Kang HAN ¹ ; Xiao-Fang WU ; De-Shun XU ; Li-Ping CHEN ; Lei JI
Author Information

1. 313000,湖州市疾病预防控制中心
Keywords: Norovirus; Reverse transcriptase polymerase chain reaction; Specificity and sensitivity
From: Chinese Journal of Experimental and Clinical Virology 2013;27(5):379-381
CountryChina
Language:Chinese
Abstract: Objective The purpose of this study was to develop RT-PCR assay for Rapidly detect and distinguish between Norovirus genogroup Ⅰ and genogroup Ⅱ with a pair of primers.Methods A pairs of primers specific to capsid prote in ORF2 gene of G Ⅰ and G Ⅱ Norovirus were dsigned according to the published complete genome sequence,with which the RNA of Norovirus was extracted and RT-PCR amplification.The sensitivity,specificity of the RT-PCR assay was estimated and apply it to the detection of Norovirus in clinical specimens.Results The results showed that the assay possessed high specificity for Norovirus detection and without any evident cross-reaction with other viruses,including rotavirus,enteric adenovirus and hepatitis A virus.The detection limit of RT-PCR assay for Norovirus G Ⅰ and G Ⅱ were up to 100 pg/ml and 10 pg/ml respectively.Conclusion The RT-PCR assay provide rapid and sensitive detection of Norovirus G Ⅰ and G Ⅱ and should prove to be useful for Norovirus diagnosis in the outbreaks of acute gastroenteritis.