Low-intensity pulsed ultrasound prompts tissue-engineered bone formation after implantation surgery.

Juyong Wang; Juqiang Wang; Asou Yoshinori; Fu Paul; Huiliang Shen; Jiani Chen; Shinichi Sotome; Zhao Liu; Kenichi Shinomiya

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Low-intensity pulsed ultrasound prompts tissue-engineered bone formation after implantation surgery.

Author: Juyong WANG ¹ ; Juqiang WANG ² ; Asou YOSHINORI ³ ; Fu PAUL ⁴ ; Huiliang SHEN ⁵ ; Jiani CHEN ³ ; Shinichi SOTOME ⁶ ; Zhao LIU ¹ ; Kenichi SHINOMIYA ⁶ ;
Author Information

1. Department of Orthopaedic Surgery, Xuanwu Hospital, Capital Medical University, Beijing 100053, China.
2. Department of Orthopaedics, First Hospital of China Medical University, Shenyang, Liaoning 110001, China.
3. Department of Orthopaedic Surgery, Graduate School, Tokyo Medical and Dental University, Tokyo 1138519, Japan.
4. Department of Neurological Surgery, Wayne State University School of Medicine, Detroit, MI 48201, USA.
5. Department of Orthopaedic Surgery, Xuanwu Hospital, Capital Medical University, Beijing 100053, China. Email: wangjywh@yahoo.com.
6. Department of Orthopaedic Surgery, Graduate School, Tokyo Medical and Dental University, Tokyo 1138519, Japan
Publication Type:Journal Article
MeSH: Animals; Bone Marrow Cells; physiology; Bone Transplantation; Calcium Phosphates; pharmacology; Male; Osteogenesis; physiology; Rats; Stromal Cells; Tissue Engineering; methods; Transplantation, Isogeneic; Ultrasonics; methods
From: Chinese Medical Journal 2014;127(4):669-674
CountryChina
Language:English
Abstract:
BACKGROUNDA practical problem impeding clinical translation is the limited bone formation seen in artificial bone grafts. Low-pressure/vacuum seeding and dynamic culturing in bioreactors have led to a greater penetration into the scaffolds, enhanced production of bone marrow cells, and improved tissue-engineered bone formation. The goal of this study was to promote more extensive bone formation in the composites of porous ceramics and bone marrow stromal cells (BMSCs).
METHODSBMSCs/β-tricalcium phosphate (β-TCP) composites were subcultured for 2 weeks and then subcutaneously implanted into syngeneic rats that were split into a low-intensity pulsed ultrasound (LIPUS) treatment group and a control group. These implants were harvested at 5, 10, 25, and 50 days after implantation. The samples were then biomechanically tested and analyzed for alkaline phosphate (ALP) activity and osteocalcin (OCN) content and were also observed by light microscopy.
RESULTSThe levels of ALP activity and OCN content in the composites were significantly higher in the LIPUS group than in the control group. Histomorphometric analysis revealed a greater degree of soft tissue repair, increased blood flow, better angiogenesis, and more extensive bone formation in the LIPUS groups than in the controls. No significant difference in the compressive strength was found between the two groups.
CONCLUSIONLIPUS treatment appears to enhance bone formation and angiogenesis in the BMSCs/β-TCP composites.