Transient receptor potential melastain 7 channel protein in human head and neck carcinoma cells and role in cell proliferation
10.3321/j.issn:1673-0860.2008.06.013
- VernacularTitle:头颈部鳞状细胞癌瞬时受体电位M7通道蛋白与肿瘤细胞增生的关系
- Author:
Jie JIANG
1
;
Wen-Bin LEI
;
Jian-Bo SHI
;
Zhen-Zhong SU
;
Zhi-Gang XIONG
Author Information
1. 广东省人民医院
- Keywords:
Ion channels;
Tumor ceils,cultured;
Carcinoma,squamous cell;
Head and neck neoplasms
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2008;43(6):451-455
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the presence of ion channel protein and its role in cell growth and proliferation in human head and neck squamous carcinoma ceils(SCC).Methads Human head and neck squamous carcinoma SCC-25 cell line was tasted with transient receptor potential melastatin 7(TRPM7) antibody using the method of immunocytochemistry.The role of TRPM7 in cell growth and proliferation was evaluated through its blockade by ion channel blockers and specific siRNA using lactate dehydrogenase (LDH)assay technique.Results Clear immunoreactivity against TRPM7 was detected in almost all SCC-25 cells tested,whereas no immunoreactivity was observed in negative contr01.The inhibitory effect of Gd3+,a non-specific ion channel blocker,on cell growth and proliferation was potenL Addition of growth of SCC-25 cells,as compared with control cells growing in normal medium(t was 4.1414 and 6.2661.P was 0.0256 and 0.0082 respectively).However,the effect of 2-APB was striking.Cell n=16)compared with cells growing in normal medium.Suppression of TRPM7 expression by siRNA also significantly inhibited the growth and proliferation of these cells(t=4.3446,P=0.0002,n=32,compared) with nontransfected ceHs).whereas cells transfected with negative control siRNA showed no difierence in cell proliferation compared with nontransfected cells.Conclusions All of those results strongly suggest the existence of TRPM7 channel in human head and neck squamous carcinoma cells.Ion channel blockers serve as a potent inhibitor of SCC-25 cell proliferation.The striking inhibitory effect of 2-APB on cell growth and proliferation may promise clinical workers an inspiring remedy for fighting against carcinoma.
