Effects of minimal persistent inflammation on nasal mucosa of experimental allergic rhinitis in guinea pigs
10.3321/j.issn:1673-0860.2008.07.005
- VernacularTitle:实验性变应性鼻炎最轻持续炎性反应对豚鼠鼻黏膜的影响
- Author:
Fei LEI
1
;
Ya-Nan CUI
;
Yun-Peng JIANG
;
Hong KONG
;
Dong-Dong ZHU
;
Zhen DONG
Author Information
1. 吉林大学中日联谊医院
- Keywords:
Rhinitis,allergic,seasonal;
Models,animal;
Transforming growth factor beta;
Gelatinsase B
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2008;43(7):499-503
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an animal model of minimal persistent inflammation(MPI)of allergic rhinitis in guinea pigs and to investigate the changes of nasal mucosa.The expression of transforming growth factor-β1(TGF-β1)and matrix metalloproteinases-9(MMP-9)were discussed. Methods Thirty male Hartley guinea pigs were randomly divided into two groups:MPI model group and control group randomly,with fifteen animals in each group.Guinea pigs from MPI model group were sensitized intraperitoneally by injection of suspension of ovoalbumin(OVA)and aluminum hydroxide in 0.9%physiological saline.Then.repeated local booster sensitization with low concentration of OVA suspension into the nasal cavity was performed to establish MPI models.Alcian blue-periodic acid-Schiff(AB-PAS)staining and Masson's trichrome(MT)staining were used to determine the number of goblet cells and collagen deposition within the basement membrane of epithelium.The expression and distribution of TGF-β1 and MMP-9 in nasal mucosa were estimated by double immunofluorescence under a confocal laser scan microscopy system.Results Compared with the control group,the increased goblet cells(t=13.720,P＜0.05)in nasal epithelium together with the increased collagen fibrils(t=4.542,P＜0.05)within the basement membrane of epithelium were observed in the MPI model group.There was nearly no expression of TGF-β1 in the control group and the expression of MMP-9 was only found in the epithelium cell.In contrast,there was significantly higher expression of TGF-β1 and MMP-9(t=25.218,P＜0.05)in nasal mucosa of MPI model group than that in control group.TGF-β1 mainly expressed in the epithelium cell,the infiltrated inflammatory cell and extracellular matrix,while MMP-9 expressed in the epithelium cell and the infiltrated inflammatory cell.Conclusions Long time MPI in allergic rhinitis resulted in some changes of tissue remodeling in nasal mucosa.TGF-β1 and MMP-9 may play an important role in disease progression.
