Expression and significance of galectin-3 and galectin-9 in mice nasal mucosa with allergic rhinitis
10.3321/j.issn:1673-0860.2008.07.006
- VernacularTitle:变应性鼻炎小鼠鼻黏膜中半乳糖凝集素3和9的表达
- Author:
Jia-Li HAN
1
;
Zhong REN
;
Xue-Jin JIANG
Author Information
1. 中国医科大学附属第一医院
- Keywords:
Rhinitis,allergic,seasonal;
Models,animal;
Galectins
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2008;43(7):504-508
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the expression of galectin-3 and galeetin-9 in mice nasal mucosa,and to explore the role of galectin-3 and galectin-9 in allergic rhinitis(AR).Methods Twenty mice were randomly divided into AR group and control group,10 mice in each group.Ten mice of BALB/c were sensitized intraperitoneally with 10 μg of ovalbumin(OVA)adsorbed onto 2 mg Al(OH)3 on day 1 and day 14.Mice was induced daily by intranasal daily administration of 10 μl of saline containing 100 μg of OVA from day 21 to day 28.OVA Was replaced with saline in control group.Immunohistochemical staining was used to examine the expression of galectin-3 and galectin-9 in Hasal mucosa.RT-PCR was performed to investigate the level of mRNA expression of complement galectin-3,galectin-9.The level of IL-4,IL-5 and IFN-γ in peripheral blood were titrated by ELISA.Results Galectin-3 and galectin-9 were detected in both groups.Expression of galectin-3 and galectin-9 in group AR was higher than that in control group.Pearson correlation analysis demonstrated that the levels of galectin-3 and galectin-9 were positivly correlaed with the level of IL-4 and IL-5,but negativly correlaed with the level of IFN-γ.Conclusions Galectin-3 and galectin-9 might play an important role in the pathogenesis of allergic rhinitis.
