Analysis of the main components of inner ear antigens inducing autoimmune Meniere's disease in guinea pigs
10.3321/j.issn:1673-0860.2008.08.008
- VernacularTitle:豚鼠自身免疫性梅尼埃病模型的主要内耳抗原分析
- Author:
Ling LU
1
;
Chang-Qiang TAN
;
Yu-Gui CUI
;
Gui-Peng DING
;
Xiao-Bin JU
;
Yu-Jin LI
;
Wen-Jun CAI
Author Information
1. 南京医科大学附属第一医院
- Keywords:
Endolymphatic hydrops;
Models,animal;
Autoimmunity;
Antigens
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2008;43(8):596-600
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the main components of inner ear antigens inducing autoimmune Meniere's disease(AIMD) in guinea pigs. Methods The guinea pigs were immunized with isologous crude inner ear antigens (ICIEAg). Then, the hearing function was measured with auditory brainstem response (ABR), the vestibular function was measured with electronystagmography (including spontaneous nystagmus and caloric test), and inner ear histopatholoical changes were observed by inner eareelloidin section with haematoxylin-eosin staining and observed under light microscope. According to these results, the AIMD-model animals from non-AIMD-model ones were distinguished. The special antibodies against ICIEAg in sera were measured with ELISA. The antigen-antibody reactions against different components of ICIEAg were detected by Western blotting with sera of AIMD and non-AIMD guinea pig srespectively. Then, we analysed the contrast between them and found the main components of the ICIEAgthat were positive reaction in AIMD guinea pigs and negative reaction in non-AIMD guinea pigs. Results The result of ELISA demonstrated that the sera of both the AIMD and non-AIMD guniea pigs contained thespecial antibodies against ICIEAg after immunized with ICIEAg. The difference of the amount of antibodyagainst ICIEAg between AIMD guinea pig group and non-AIMD guinea pig group was not significant. Western blotting assay showed only the sera of AIMD guinea pig contained the antibodies against the specific antigens with the molecular of 68 000, 58 000, 42 000 and 28 000. Conclusions ICIEAg contain many different components, the AIMD might only happen in the guinea pigs in which the special immunization against themain components that could induce this kind of disorder appeared. The inner ear antigens with molecular of68 000, 58 000, 42 000 and 28 000 might be the main components inducing AIMD in guinea pigs.
