Expression of surviving and easpase-3 during the development process in rat cochlea
10.3321/j.issn:1673-0860.2008.09.011
- VernacularTitle:大鼠耳蜗发育过程中生存素和半胱氨酸天冬氨酸蛋白酶3的表达
- Author:
Chun-Hui LI
1
;
Bo DU
;
Ping WANG
;
Bao-Dong DU
;
Feng-Lan ZHANG
Author Information
1. 吉林大学第二医院
- Keywords:
Cochlea;
Organogenesis;
Survivin;
Caspase-3;
Apoptosis
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2008;43(9):686-690
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of xome apoptosis regulators during the development in rat cochlea.Methods The morphological development process of cochlea was obserred in Wistar rat aged between embryo day 13 to postnatal day 14 in this experiment.Survivin and caspase-3 Were respectively detested at protein and mRNA levels by immunohistochemistry and reverse transcription polymerage chain reaction(RT-PCR).Results The expression of survivin and cagpage-3 located in the bottom wall of the cochlear duct.Not only they widespread in the cell proliferation,but also they gyadually enhanced in the cell differentiation.Both of them had a crest-time.and survivin was prior to caspase-3.In organ of corti during adult time,caspase-3 Wag not present and survivin was only expressed.Conclusions During the development of the rat cochlea,both of them had similar location and trend.But they were derangement.This showed that beth of them participated in the cochlear morphological development.It wag suggested that the interaction between survivin and caspas-3 regulated the apoptosis,promoted the cochlear morphological development.
