Global gene expression profiling of laryngeal squamous cell carcinoma by laser capture microdissection and complementary DNA microarrays
10.3321/j.issn:1673-0860.2008.09.013
- VernacularTitle:激光捕获显微切割及基因芯片技术构建喉鳞状细胞癌全基因组表达谱
- Author:
Li-Juan MA
1
;
Yong-Quan TIAN
;
Jian-Yun XIAO
;
Wei LI
;
Hua ZHANG
;
Xin ZHANG
;
Dong-Hai HUANG
Author Information
1. 中南大学湘雅医院
- Keywords:
Laryngeal neoplasms;
Carcinoma,gquamous cell;
Oligonucleotide array sequence analysis;
Laser capture microdissection
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2008;43(9):696-700
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the gene expression profile of laryngeal squamous cell carcinoma (LSCC) by combination of laser capture micmdissection (LCM) and microarray and to identify genetic changes in disease pathogenesis.Methods The study analysed 8 matched pairs of specimens of glottic carcinoma of larynx and histologically normal epithelium tissues adjacent to the carcinoma preserved in the RNAlater reagent.A genome-wide transcriptome analysis was performed by probing 16 cDNA microarrays with fluorescent-labeled amplified RNA derived from higer capture microdissected cells.Real-time quantitative (RT-PCR) of tissue mieroarmy was used to validate the reliability of cDNA miemarrays.Results Significant analysis of microarray(SAM) floftware and hierarchical cluster analysis of the expressed genes showed that 2351 genes was signiilcantly expressed respectively according to different analysis method (false discover rate=0.63%).A selected set of MMP12,KRT16,RARB,PRB1 genes was identified to be consistent with array data by RT-PCR. Condusiom The analysis of gene ontology and pathway distributions futher highlighted genes that may be critically important to laryngeal carcinogenesis.The results strongly suggest that this new approach may facilitate the identification of clinical molecular markers of disease and novel potential therapeutic targets for LSCC.
