Expression of full-length spleen tyrosine kinase in the oral squamous cell carcinoma and its relationship with tumor invasion and metastasis.
- Author:
Zhao WANG
;
Jie CHEN
;
Weiming CHU
;
Mingjie DA
;
Lu MA
;
Min WU
;
Yi ZHONG
;
Zilu WANG
;
Xiaomeng SONG
;
Yunong WU
- Publication Type:Journal Article
- MeSH: Blotting, Western; Carcinoma, Squamous Cell; metabolism; Humans; Immunohistochemistry; Lymphatic Metastasis; Mouth Neoplasms; metabolism; RNA, Messenger; Syk Kinase; metabolism
- From: West China Journal of Stomatology 2015;33(5):519-523
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of full-length spleen tyrosine kinase [SYK (L)] mRNA and protein in human oral squamous cell carcinoma (OSCC) as well as its possible effects on the invasion and metastasis of OSCC.
METHODSThe expression of SYK (L) was detected in 27 cases of OSCC tissues and its matched adjacent non-cancerous tissues by real-time quantitative polymerase chain reaction (RT-qPCR), Western blot, and immunohistochemistry. Fourteen cases of normal oral gingival tissues were also analyzed as a normal control.
RESULTSReduced mRNA and protein expression of SYK (L) in OSCC tissues was observed compared with that in normal oral gingival tissues (P<0.01) and adjacent non-cancerous tissues (P<0.05). SYK(L) expression was significantly associated with lymph-node metastasis (P<0.05).
CONCLUSIONSYK(L) is a candidate tumor suppressor for OSCC tissues, and has an inhibitive effect on the initiation, proliferation, and lymph-node metastasis of human OSCC.